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Cells were incubated with primary antibodies for 24 h at room temperature while rotating, with dilutions ranging from 1:50 to 1:200, followed by a 1-h incubation with a species-specific fluorophore-conjugated secondary antibody diluted in 3% BSA and 0.1% Tween-20 in 1 PBS

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Numerous factors, including the geographic location, the number of samples and the inspection process, may be responsible for the virus spreading at this rate in comparison to other nations